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Image Search Results
Journal: Neurobiology of learning and memory
Article Title: Female Mice with Apolipoprotein E4 Domain Interaction Demonstrated Impairments in Spatial Learning and Memory Performance and Disruption of Hippocampal Cyto-Architecture
doi: 10.1016/j.nlm.2019.03.012
Figure Lengend Snippet: (A) Representative 40x image of the different types of Calretinin-positive (CR+) neurons that were counted. CR+ cells stained with CY3 (red) are widespread in the hippocampus. The cells co-express NeuN stained with 488 (green). The larger CR+ cells are mostly found in the hilus (marked with the yellow x) and they are mossy cells. The smaller CR+ cells which are located between the granular cell layer (GCL) and the hilus (indicated by white arrowheads) are newborn neurons being added to the GCL. Scale bar 50μm. (B) Representative images of CR and NeuN double-staining in the hippocampus; note the higher number of CR+ neurons especially in the young Arg-61 apoE mice (B-iii). (C) Stereology data showing no difference of CR+ cells in the hilus in any of the groups. (D) The number of CR+ cells in sub granular zone were higher in 5 month, but in a trend of lower (p = 0.056) in 10month Arg-61 apoE mice versus age-matched C57BL/6J mice. N=4 for all groups except 10-month Arg-61 mice where N=3. Data was analyzed by 2-way ANOVA followed by Tukey’s multiple comparison post-hoc test. **p<0.01, ***p<0.001.
Article Snippet:
Techniques: Staining, Double Staining